Subcellular Targeted Metabolomics Service

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Subcellular Targeted Metabolomics Service

Subcellular Targeted Metabolomics Service

Targeted metabolomics can detect and analyze specific metabolites in a specific and specific way, validate candidate biomarkers and analyze known target compounds. Targeted metabolome can enrich blood, urine or other body fluids as well as a specific metabolite in tissues and make accurate quantitative and qualitative analysis.

Creative Proteomics have gas chromatography-mass spectrometry platform, liquid chromatography-mass spectrometry platform and nuclear magnetic resonance quantitative metabolomics platform, as well as MRM (multiple reaction monitoring) and PRM (parallel reaction monitoring) technology, can provide you with targeted metabolomics analysis services. We can help you detect metabolites secreted by organelles or related products of the metabolic reactions that they participate in, thereby accelerating relevant research in biopharmaceuticals, disease mechanisms, agriculture, or microbes.

Technology Platforms

Technologies Instruments Sensitivity Characteristics
Nuclear Magnetic Resonance Spectroscopy 700 MHz Bruker Avance III HD10-6
  • Non-invasive detection.
  • Small sample requirements.
  • High specificity.
  • Qualitative and quantitative detection of metabolites can be performed simultaneously.
  • Able to obtain flat baseline and high linear response without phase distortion
  • The dynamic range is limited and the sensitivity is low.
GC-MS Finnigan TRACE DSQ GC-MS system with Electron Ionization (EI) and Chemical Ionization (CI) capability; 7200B GC/Q-TOF10-12
  • High sensitivity.
  • The separation effect is good.
  • The database is relatively sound.
  • Troublesome sample processing
  • Difficult or semi-volatile substances need to be derivatized or use LC-MS.
LC-MS Agilent Ion Trap LC-MS system10-15
  • High sensitivity and high resolution
  • Metabolites that are unstable, difficult to derive, difficult to volatilize and have large molecular weight can be analyzed

Advantages of Targeted Metabolomics Analysis Service:

  • Advanced instruments and equipment: Creative Proteomics has a variety of detection equipment such as NMR, LC-MS, GC-MS, and can be flexibly selected according to different experimental needs.
  • Rich experience: Creative Proteomics has 13 years of experience in proteomics and metabolomics analysis, and has a professional metabolomics team.
  • Strict quality control: From sample processing, quality evaluation to machine testing, every step is strictly controlled to ensure the accuracy of the results.
  • Professional bioinformatics analysis: Creative Proteomics has a professional and experienced bioinformatics analysis team to provide you with comprehensive and high-quality data analysis.
  • Cost-effective: Creative Proteomics can provide customers with comprehensive services to help you effectively save experimental costs.

Sample Requirements

Sample Types Sample Volume Notes
Tissues (Plants and Animals)≥100 mgThe minimum weight should not be less than 50 mg.
Cells and Microbes Samples≥10 mg; Bacterial Solution ≥20 µL; Number of cells ≥107It must be quenched. Need to quickly inactivate microorganisms with liquid nitrogen or organic solvents to stop their metabolism instantly
Biological Fluid (Plasma, Serum)≥200 µLRepeated freezing and thawing of sample must be eliminated.  The total volume is not less than 100 µL. Plasma is the fluid that removes blood cells after anticoagulation with an anticoagulant. Serum is the supernatant after blood clotting. Stored at -80°C.

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*For Research Use Only. Not used for clinical diagnosis and treatment.

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