Exosomes Identification Service
Exosomes are involved in the pathological process of many diseases such as neurological diseases and cancer. At the same time, they also play an important function of mediating intercellular communication in normal physiological processes. Because they are rich in biomarkers, they can be used to monitor disease progression, treatment response, etc. Exosomes can also carry and deliver biomolecules and have the potential to become clinical drug delivery vehicles. In order to better study the function of exosomes, the first step is to identify exosomes.
The isolated exosomes need to be identified from three levels:
(1) Western blot (WB) to identify the surface markers of exosomes;
(2) Transmission electron microscopy (TEM) to identify the morphology of exosomes;
(3) Particle size analysis (NTA) to identify the size of exosomes.
The identification of exosomes includes the identification of protein components and nucleic acid components. Protein components: cytoskeleton proteins, signal transduction related proteins, metabolic enzymes, antigen binding and presentation related proteins. Nucleic acid components: Exosomes can also encapsulate mRNA, miRNA, lncRNA, mtDNA, circRNA, and transfer to other cells to play a biological role. The contents of Exosomes are also cell type specific.
Creative Proteomics aims to provide high-quality proteomics-related services to customers in different fields. Our scientists have developed an exosome identification platform based on professional knowledge and advanced equipment to help accelerate the progress of your project.
Technology platform of exosomes identification service
- WB verification: Detect the expression of exosomal marker proteins.
- Electron microscope: Analyze the size/morphology of exosomes, etc.
- Nanoparticle tracking analysis (NTA): Detect the size and concentration of exosomes.
- Flow cytometry: Detect biomarkers of exosomes.
- Mass spectrometry: Qualitative and quantitative analysis of proteins.
To identify exosomes, WB must be used to identify whether the marker protein of exosomes is present in the sample. Exosomal membranes are rich in four transmembrane protein families (CD63, CD81 and CD9) involved in exosomal transport, heat shock protein families ((HSP60, HSP70, HSPA5, CCT2 and HSP90) and some cell-specific proteins Including A33 (derived from colonic epithelial cells), MHC-II (derived from antigen-presenting cells), CD86 (derived from antigen-presenting cells) and lactectin (immature dendritic cells). Among them, CD63, CD9, CD81 and TSG101 , HSP70, ALIX, etc. are the most commonly used extracellular vesicle markers.
Scanning electron microscopy (SEM) or transmission electron microscopy (TEM) and other electron microscopes have high resolution and can directly observe the morphology of exosomes in the sample and identify exosomes of different sizes.
Compared with other characterization methods, NTA technology has simpler sample processing, better guarantees the original state of exosomes, and faster detection speed.
Flow cytometry detection technology is relatively fast, suitable for high-throughput screening, and can analyze the size and volume of particles. Use antibodies corresponding to specific markers on the surface of exosomes for labeling, and then use flow cytometry to detect their positive expression to identify exosomes.
Identification and quantitative analysis of proteins in exosomes by high-resolution mass spectrometry.
Other related services:
*For Research Use Only. Not used for clinical diagnosis and treatment.