Nuclear Proteomics Services

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Nuclear Proteomics Services

Nuclear Proteomics Services

The nucleus is the storage place of genetic information, which is responsible for gene replication, transcription, and processing of transcript products, and is also the regulatory center of cellular genetic and metabolic activities. According to different research purposes and methods, Creative Proteomics provides a one-stop service to comprehensively analyze nuclear proteomics from a holistic perspective. The main contents include: 1) separation and purification of nuclear and nuclear proteins; 2) study on the expression of nuclear proteins; 3) structural studies of nuclear proteins; 4) functional research of nuclear proteins.

We Provide the Following Services, including but not Limited to:

  • Nucleus Isolation and Nuclear Protein Purification
    • Plant nuclei: Nuclei are mainly extracted from plant seedlings or suspension culture cells. Nuclei were enriched by Percoll density gradient centrifugation and verified for purity. Nuclear protein was extracted by phenol extraction or TCA acetone precipitation and verified for purity.
    • Animal cell nuclei: Animal cells can be broken with homogenizer, tissue disperser, ultrasonic, dry acetone powder and other methods. Nuclei are enriched by high-speed centrifugation with sucrose density gradient and purity verified.
  • Study on Dynamic Changes of Proteins in Cell Nucleus
  • Fluorescence recovery and fluorescence loss after bleaching are often used. Some proteins are mobile in the nucleus and can quickly bind and dissociate with their respective regions. And this kind of movement is mainly carried out in a passive diffusion manner, without consuming metabolic energy. In addition, covalent modification of proteins can affect the movement of certain proteins. Studying the dynamic changes of proteins in the nucleus is helpful for studying the structural composition of the nucleus and the regulation of gene expression.

  • Study on Nuclear Protein Expression
    • Quantitative analysis of nuclear proteins
    • Qualitative analysis of nuclear proteins
    • Study differentially expressed proteins
  • Study on the Structure of Nuclear Proteins
  • Analysis of Protein Advanced Structure

  • Functional Research of Nuclear Proteins
  • The purpose of nuclear functional proteomics is to study the function of intracellular nuclear proteins. Target groups of proteins with specific functional mechanisms. Research contents include the regulation of transcription factors, glycosylation and phosphorylation of proteins, protein interactions and research on signal transduction pathways, etc.

    • Functional proteome in the nuclei
    • Localization of functional proteome in nuclei
    • Functional proteome participates in signaling pathways in the nuclei

In addition, Creative Proteomics also provides two detailed services:

Nuclear Proteomics Technology Roadmap

Nuclear proteomics technology roadmap

Data Analysis of Nuclear Proteomics Services

1. Data output statistics and quality control2. Protein identification analysis (Density map, Scatter plot, Venn diagram)3. Quantitative protein analysis (Density map, Scatter plot, Venn diagram)
4. Protein GO analysis5. Protein pathway analysis6. Protein COG analysis
7. GO enrichment analysis of differential proteins8. Pathway enrichment analysis of differential proteins9. COG analysis of differential proteins

References

  1. ang J, Mauvoisin D, et al. Nuclear proteomics uncovers diurnal regulatory landscapes in mouse liver. Cell metabolism, 2017, 25(1): 102-117.
  2. Yin X, Komatsu S. Plant nuclear proteomics for unraveling physiological function. New biotechnology, 2016, 33(5): 644-654.

*For Research Use Only. Not used for clinical diagnosis and treatment.

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