Pancreatic Cancer Exosome Research Solution
Online InquiryPancreatic cancer (PC) is a recalcitrant and serious disease due to its complex and latent pathogenesis and the lack of early diagnostic methods. Mortality from PC is rapidly increasing and is expected to be the second most common of all malignancies by 2030. Therefore, researchers are working to investigate the biological mechanism of PC occurrence and development and to develop markers to screen for early curable pancreatic lesions. Increasing evidence suggests that exosomes play a crucial role in tumorigenesis and progression. In recent years, the widespread use of exosomes as biomarkers and drug carriers has made them attractive in the field of PC.
Fig. 1 Various effects of exosomes on PC progression. (Guo, Xiao-Yu, et al., 2019)
Role of exosomes in PC
Exosomes are nanosized, lipid bilayer membrane-enclosed extracellular vesicles (EVs) ranging in diameter from 30 to 150. These EVs contain functional biomolecules, such as proteins, nucleic acids, and metabolites and can be secreted by a wide range of cell types and cancer cells. Exosomes, as important information transporters between cells, facilitate cell-cell and cell-microenvironment communication in both normal and malignant cells. In the past decade, there has been an increasing number of exosome-related studies that have demonstrated the involvement of exosomes in a variety of cancer-related processes, including tumor cell proliferation, invasion and metastasis, apoptosis inhibition, chemoresistance, and immune tolerance.
| Functions of exosomes in pancreatic cancer (PC) | |
|---|---|
| Specific role | Function description |
| PC cell proliferation | Different sources of exosomes have different or even opposite effects on PC cell proliferation, which needs further elucidation and in-depth study. |
| PC invasion and metastasis | Exosomes may promote cancer invasion and metastasis by regulating stromal cells, remodeling the extracellular matrix (ECM), and stimulating angiogenesis. |
| Formation of PC drug resistance | Exosomes from the PC or tumor microenvironment can promote drug resistance of PC cancer cells through regulating proteins, associated genes, RNA, and signaling pathways. |
Applications of exosomes in PC
- Exosomes as biomarkers for non-invasive diagnosis of PC, including diagnosis and prognosis
- Exosomes as therapeutic targets of PC
- Exosomes as a therapeutic vehicle of PC
Pancreatic cancer exosome research solution at Creative Proteomics
Exploring the biological mechanisms underlying the development of PC will provide a direction for its prevention and treatment. Our experts with years of experience in exosome research can help you plan, conduct, and accelerate your projects. Creative Proteomics provides the following exosome-based services to support PC exosome research, including but not limited to:
| Exosome analysis services | |
|---|---|
| Exosome isolation and purification | Sucrose gradient centrifugation |
| Polymer-based exosome enrichment method | |
| Immunomagnetic bead method | |
| Size exclusion chromatography method | |
| Exosome identification | Nanoparticle tracking analysis (NTA) |
| Electron microscopy analysis | |
| Western blot | |
| Exosome marker assay | Isolation and enrichment of exosomal CD9, CD63, CD81, TSG101, HSP70 proteins |
| Exosome surface protein identification and quantitative analysis | |
| Exosome engineering | Exosome labeling and tracking |
| Cargo loading | |
| Engineered exosome production | |
| Cargo loading assessment | |
| Exosomes labeling and tracking | Exosome fluorescent labeling |
| Fluorescence exosome purification | |
| Fluorescent exosome concentration labeling | |
| Exosome multiomics analysis | Exosome proteomics analysis: Exosome protein profile identification Exosome protein composition analysis Exosome protein expression level analysis Exosome protein differential expression analysis |
| Exosome metabolomics analysis: Exosome differential metabolite screening Qualitative and quantitative analysis of target metabolites/metabolic pathways | |
| Exosome lipidomics analysis: Exosome lipid composition and level analysis Differential expression analysis of exosomal lipid molecules Qualitative and quantitative analysis of targeted lipid molecules | |
| Exosomal biogenesis and identification | Our capabilities will provide strong support for the study of exosome biogenesis and its identification. |
| Exosomal cargo and loading mechanism | Our services can greatly help the study of exosomal cargo and loading mechanism, facilitating the diagnostic and therapeutic applications of exosomes. |
| Exosome function research | In vitro analysis of the function of exosomes In vivo analysis of the function of exosomes |
Advantages of our services
- Preparation of high-purity exosomes
- High-performance mass spectrometry (MS) system
- Comprehensive bioinformatics analysis
Creative Proteomics offers a reliable, rapid, and cost-effective one-stop solution for multiple cancer exosome research based on highly stable, reproducible, and sensitive systems for isolation, characterization, identification, and quantitative analysis. Thank you for your interest in our services. Let us know what you are trying to achieve and our team of experts will be in touch with you within one business day to discuss your needs.
References
- Lan, Bin, et al. "The role of exosomes in pancreatic cancer." International Journal of Molecular Sciences 20.18 (2019): 4332.
- Guo, Xiao-Yu, et al. "Exosomes and pancreatic diseases: status, challenges, and hopes." International Journal of Biological Sciences 15.9 (2019): 1846.
* For Research Use Only. Not for use in diagnostic procedures.
