Nuclear Pore Complex Proteomics Services

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Nuclear Pore Complex Proteomics Services

Nuclear Pore Complex Proteomics Services

Nuclear pore complex (NPC) mediates the shuttle movement of macromolecules between the cytoplasm and the nucleus. NPC is located in the nuclear membrane, forming a hydrophobic channel. It enables macromolecular substances such as proteins to bind to transport receptors for transmembrane transport. This transmembrane transport through the nuclear pore complex plays an important physiological function in life activities such as cell proliferation, cell differentiation, and individual development.

Creative Proteomics provides a one-stop service for nucleolar proteomics, and conducts a comprehensive analysis of nucleolar proteomics from a holistic perspective. The main contents include: 1) Study the structural composition and dynamic changes of nuclear pore complexes; 2) Study the role of nuclear pore complexes in life activities such as embryonic development, gene transcription and translation, cell differentiation, and apoptosis; 3) Study the trans-nuclear membrane transport mode of macromolecular substances; 4) Study the regulatory mechanism of matter through the nuclear pore complex; 5) Data analysis and bioinformatics analysis.

We Provide the Following Services, including but not Limited to:

  • Study on the Structure and Dynamic Changes of Nuclear Pore Complex
  • 1. Study the formation of nuclear pores
    2. Study the location of nuclear pore complexes
    3. Investigate the structural composition of nuclear pore complex
    4. Investigate the conformational changes of NPC that small or large molecules may cause through the nuclear pore complex.
    5. Investigate the role of cytoplasmic and nucleoplasmic loops in the process of material transport.

  • Physiological Function of Nuclear Pore Complex
  • 1. Study on the function of nuclear pore complex characteristic proteins. For example: nuclearlamin, emerin
    2. Investigate the role of nuclear porin components in spindle orientation and mitosis
    3. Investigate the role of nuclear porin complexes in life development such as embryo development, gene transcription and translation, cell differentiation, and apoptosis
    4. The connection between nuclear pore complex and aging

  • Role of Nuclear Pore Complex Proteins in Molecular Transmembrane Transport
  • 1. Trans-nuclear membrane transport mode of macromolecular substances
    Study nuclear translocation signals and transport vectors which contain importin, exportin, and transportin.
    2. Regulation mechanism of substances passing through nuclear pore complex

  • Bioinformatics Analysis
  • For example, nucleolar protein domain function analysis, homology analysis and evolution analysis.

Advantages of the Nuclear Pore Complex Proteomics Services:

  • One-stop service: we will customize a solution for you based on your samples and needs. You can also choose which technique to use for analysis.
  • High performance mass spectrometry system: equipped with Q Exactive Hybrid Quadrupole-Orbitrap and Agilent 6540 Q-TOF high performance mass spectrometer which effectively improving the identification and quantification of low abundance subcellular related proteins.
  • Multi-database integration: by integrating multiple related high-quality protein databases, the accuracy and flux of subcellular protein localization can be greatly improved.
  • Multi-dimensional data analysis: in addition to the comparison with the more complete related subcellular database, multiple software was used for the prediction analysis of subcellular localization based on the calculation method to further increase the identification flux.

Nuclear Pore Complex Proteomics Technology Roadmap

Nuclear pore complex proteomics technology roadmap

Data Analysis of Nuclear Pore Complex Proteomics

1. Data output statistics and quality control2. Protein identification analysis (Density map, Scatter plot, Venn diagram)3. Quantitative protein analysis (Density map, Scatter plot, Venn diagram)
4. Protein GO analysis5. Protein pathway analysis6. Protein COG analysis
7. GO enrichment analysis of differential proteins8. Pathway enrichment analysis of differential proteins9. COG analysis of differential proteins

Other Related Services:

References

  1. Cronshaw J M, Krutchinsky A N, et al. Proteomic analysis of the mammalian nuclear pore complex. The Journal of cell biology, 2002, 158(5): 915-927.
  2. Alber F, Dokudovskaya S, et al. The molecular architecture of the nuclear pore complex. Nature, 2007, 450(7170): 695-701.
  3. Knockenhauer K E, Schwartz T U. The nuclear pore complex as a flexible and dynamic gate. Cell, 2016, 164(6): 1162-1171.

*For Research Use Only. Not for use in diagnostic procedures.

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